‘Seeding’ property provides new focus for treatment to delay progression of disorder
By identifying in spinal fluid how the characteristic mutant proteins of Huntington’s disease spread from cell to cell, UC Irvine scientists and colleagues have created a new method to quickly and accurately track the presence and proliferation of these neuron-damaging compounds – a discovery that may accelerate the development of new drugs to treat this incurable disease.
The researchers added that the cell-to-cell “seeding” property of these mutant proteins seems to be a critical part of the disease’s progression. Their findings also advance a new drug-discovery approach: stopping the cellular transfer of the seeding compounds. Study results appear online in Molecular Psychiatry, at http://dx.doi.org/10.1038/MP.2015.81.
Huntington’s disease is a genetically based, severe neurodegenerative disorder that results in progressive motor, cognitive and psychiatric impairment and, ultimately, death. There is no effective treatment for Huntington’s, which – like many neurodegenerative diseases – is characterized by an accumulation of misfolded mutant proteins that interfere with brain function.
As part of their study, the researchers introduced a new screening test that measures mutant huntingtin protein seeding in cerebrospinal fluid, which can be obtained via a simple procedure. This assay distinguishes symptomatic Huntington’s disease subjects – who have high seeding activity – from gene carriers not yet showing symptoms – who have lower seeding activity. Fluid samples from non-HD individuals do not exhibit this seeding property.
“Determining if a treatment modifies the course of a neurodegenerative disease like Huntington’s or Alzheimer’s may take years of clinical observation,” said study leader Dr. Steven Potkin, UCI professor of psychiatry & human behavior. “This assay that reflects a pathological process can play a key role in more rapidly developing an effective treatment. Blocking the cell-to-cell seeding process itself may turn out to be an effective treatment strategy.”
Evaluating whether a drug prevents seeding will be much quicker than longitudinal assessment of symptoms, added study co-leader Zhiqun Tan, an associate researcher at the UCI Institute for Memory Impairments and Neurological Disorders (UCI MIND). Seeding measurements can also aid in determining the optimal dosage of a new therapy. Additionally, gauging the seeding property of misfolded proteins may prove to be useful in the development of new treatments for other neurodegenerative diseases, such as Alzheimer’s, ALS and Parkinson’s.
UCI is a leading center for research on Huntington’s disease and other related neurodegenerative disorders. Its scientists have helped identify mechanisms underlying HD and are actively pursuing the creation of novel therapies.
Earlier this year, study contributor Leslie Thompson of UCI’s Sue & Bill Gross Stem Cell Research Center and UCI MIND received a $5 million grant from the California Institute for Regenerative Medicine to continue her CIRM-funded effort to develop stem cell treatments for Huntington’s disease. In this project, Thompson and her colleagues plan to create a therapy employing human embryonic stem cells that can be evaluated in clinical trials.
Theo van Erp, Julia Overman, Angelo Demuro, Michelle A. Digman, Ricardo Albay, Katya T. Potkin, Shichun Ling, Fabio Macciardi, William E. Bunney, Ian Parker and Charles Glabe of UCI; Asa Hatami and John M. Ringman of UCLA; Wah Chiu and Wei Dai of the Baylor College of Medicine; Jeffrey D. Long and Jane S. Paulsen of the University of Iowa; and Emily M. Sontag of Stanford University also contributed to the study, which received National Institutes of Health support.
Source: UC Irvine